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J Radiat Prot > Volume 33(3); 2008 > Article
Journal of Radiation Protection 2008;33(3):87-0.
마우스에서 보중익기탕이 자외선 B 조사에 의한 표피멜라닌세포 변화에 미치는 영향
이해준;김환성;박영종;김중선;문창종;김종춘;배춘식;조성기;김성호;
The Effect of Bu-Zhong-Yi-Qi-Tang on Epidermal Melanocytes in Ultraviolet B-irradiated Mice
Lee, Hae-June;Kim, Hwan-Sung;Park, Young-Jong;Kim, Joong-Sun;Moon, Chang-Jong;Kim, Jong-Choon;Bae, Chun-Sik;Jo, Sung-Kee;Kim, Sung-Ho;
ABSTRACT
We induced the activation of melanocytes in the epidermis of C57BL/6 mice by ultraviolet B (UVB) irradiation and observed the effect of Bu-Zhong-Yi-Qi-Tang (BZYQT) on the formation, and decrease of UVB-induced epidermal melanocytes. C57BL/6 mice were irradiated by UVB $80;mJ/cm^2$ (0.5 mW/sec) daily for 7 days, and BZYQT was intraperitoneally or topically applied pre- or post-irradiation. For the estimation of change of epidermal melanocytes, light microscopic observation with dihydroxyphenylalanine (DOPA) stain was performed. Split epidermal sheets prepared from the ear of untreated mice exhibited 11-16 melanocytes/$mm^2$, and one week after UV irradiation, the applied areas show an increased number of strongly DOPA-positive melanocytes with stout dendrites. But intraperitoneal or topical treatment with BZYQT before each irradiation interrupted UVB-induced pigmentation and resulted in a marked reduction in the number of epidermal melanocytes as compared to radiation control skin. The number and size of DOPA-positive epidermal melanocytes were also significantly decreased in intraperitoneally injected or topically applicated group after irradiation with BZYQT at 3rd and 6th weeks after irradiation. The present study suggests the BZYQT as inhibitor of UVB-induced pigmentation and depigmenting agent.
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